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jbs dna  (Jena Bioscience)


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    Structured Review

    Jena Bioscience jbs dna
    Jbs Dna, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/jbs+dna/JBS+DNA-Shuffling+Kit/pmc12364711-370-6-9
    Average 93 stars, based on 6 article reviews
    jbs dna - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Directed Evolution of Soluble α-1,2-Fucosyltransferase Using Kanamycin Resistance Protein as a Phenotypic Reporter for Efficient Production of 2'-Fucosyllactose
    Article Snippet: To construct a primary FucT2 mutant library, the gene coding for FucT2 was amplified with pSel2-FucT2 I FW and BW primers using a GeneMorph II Random Mutagenesis Kit (Agilent Technologies, USA) according to the manufacturer’s instructions. .. The resulting PCR products were ligated with the pSel2 plasmid to construct an intermediate plasmid, and additional mutations were introduced using a JBS DNA-Shuffling Kit (Jena Bioscience, Germany), as specified by the manufacturer. ..

    Article Title: Biosynthesis of polyketides
    Article Snippet: A thiolase library can also be a library of mutants of a certain thiolase generated through methods selected from error prone PCR random mutagenesis with usage of GeneMorph II Random Mutagenesis Kit (Agilent Technologies, Inc., Santa Clara, Calif.), site-specific saturation mutagenesis of certain sites of the gene encoding the thiolase by QuikChange Multi Site-Directed Mutagenesis Kit (Agilent Technologies, Inc., Santa Clara, Calif., USA) and DNA shuffling of the gene encoding thiolase by JBS DNA-Shuffling Kit (Jena Bioscience GmbH, Jena, Germany). .. A thiolase library can also be a library of mutants of a certain thiolase generated through methods selected from error prone PCR random mutagenesis with usage of GeneMorph II Random Mutagenesis Kit (Agilent Technologies, Inc., Santa Clara, Calif.), site-specific saturation mutagenesis of certain sites of the gene encoding the thiolase by QuikChange Multi Site-Directed Mutagenesis Kit (Agilent Technologies, Inc., Santa Clara, Calif., USA) and DNA shuffling of the gene encoding thiolase by JBS DNA-Shuffling Kit (Jena Bioscience GmbH, Jena, Germany). .. These genes are amplified from their vectors in the library through PCR using appropriate primers to append homology on each end for recombination into the vector backbone with Phusion polymerase (Thermo Scientific, Waltham, Mass.) to serve as the gene insert.

    Article Title: Biosynthesis of polyketides
    Article Snippet: .. The AraC mutant can be acquired through methods selected from error prone PCR random mutagenesis with usage of GeneMorph II Random Mutagenesis Kit (Agilent Technologies, Inc., Santa Clara, Calif.), site-specific saturation mutagenesis of certain sites of the gene encoding the thiolase by QuikChange Multi Site-Directed Mutagenesis Kit (Agilent Technologies) and DNA shuffling of the gene encoding thiolase by JBS DNA-Shuffling Kit (Jena Bioscience GmbH, Jena, Germany) on wild-type AraC. .. The AraC mutant can be acquired through methods selected from error prone PCR random mutagenesis with usage of GeneMorph II Random Mutagenesis Kit (Agilent Technologies, Inc., Santa Clara, Calif.), site-specific saturation mutagenesis of certain sites of the gene encoding the thiolase by QuikChange Multi Site-Directed Mutagenesis Kit (Agilent Technologies) and DNA shuffling of the gene encoding thiolase by JBS DNA-Shuffling Kit (Jena Bioscience GmbH, Jena, Germany) on wild-type AraC.

    Plasmid Preparation:

    Article Title: Directed Evolution of Soluble α-1,2-Fucosyltransferase Using Kanamycin Resistance Protein as a Phenotypic Reporter for Efficient Production of 2'-Fucosyllactose
    Article Snippet: To construct a primary FucT2 mutant library, the gene coding for FucT2 was amplified with pSel2-FucT2 I FW and BW primers using a GeneMorph II Random Mutagenesis Kit (Agilent Technologies, USA) according to the manufacturer’s instructions. .. The resulting PCR products were ligated with the pSel2 plasmid to construct an intermediate plasmid, and additional mutations were introduced using a JBS DNA-Shuffling Kit (Jena Bioscience, Germany), as specified by the manufacturer. ..

    Construct:

    Article Title: Directed Evolution of Soluble α-1,2-Fucosyltransferase Using Kanamycin Resistance Protein as a Phenotypic Reporter for Efficient Production of 2'-Fucosyllactose
    Article Snippet: To construct a primary FucT2 mutant library, the gene coding for FucT2 was amplified with pSel2-FucT2 I FW and BW primers using a GeneMorph II Random Mutagenesis Kit (Agilent Technologies, USA) according to the manufacturer’s instructions. .. The resulting PCR products were ligated with the pSel2 plasmid to construct an intermediate plasmid, and additional mutations were introduced using a JBS DNA-Shuffling Kit (Jena Bioscience, Germany), as specified by the manufacturer. ..

    Generated:

    Article Title: Biosynthesis of polyketides
    Article Snippet: A thiolase library can also be a library of mutants of a certain thiolase generated through methods selected from error prone PCR random mutagenesis with usage of GeneMorph II Random Mutagenesis Kit (Agilent Technologies, Inc., Santa Clara, Calif.), site-specific saturation mutagenesis of certain sites of the gene encoding the thiolase by QuikChange Multi Site-Directed Mutagenesis Kit (Agilent Technologies, Inc., Santa Clara, Calif., USA) and DNA shuffling of the gene encoding thiolase by JBS DNA-Shuffling Kit (Jena Bioscience GmbH, Jena, Germany). .. A thiolase library can also be a library of mutants of a certain thiolase generated through methods selected from error prone PCR random mutagenesis with usage of GeneMorph II Random Mutagenesis Kit (Agilent Technologies, Inc., Santa Clara, Calif.), site-specific saturation mutagenesis of certain sites of the gene encoding the thiolase by QuikChange Multi Site-Directed Mutagenesis Kit (Agilent Technologies, Inc., Santa Clara, Calif., USA) and DNA shuffling of the gene encoding thiolase by JBS DNA-Shuffling Kit (Jena Bioscience GmbH, Jena, Germany). .. These genes are amplified from their vectors in the library through PCR using appropriate primers to append homology on each end for recombination into the vector backbone with Phusion polymerase (Thermo Scientific, Waltham, Mass.) to serve as the gene insert.

    Mutagenesis:

    Article Title: Biosynthesis of polyketides
    Article Snippet: A thiolase library can also be a library of mutants of a certain thiolase generated through methods selected from error prone PCR random mutagenesis with usage of GeneMorph II Random Mutagenesis Kit (Agilent Technologies, Inc., Santa Clara, Calif.), site-specific saturation mutagenesis of certain sites of the gene encoding the thiolase by QuikChange Multi Site-Directed Mutagenesis Kit (Agilent Technologies, Inc., Santa Clara, Calif., USA) and DNA shuffling of the gene encoding thiolase by JBS DNA-Shuffling Kit (Jena Bioscience GmbH, Jena, Germany). .. A thiolase library can also be a library of mutants of a certain thiolase generated through methods selected from error prone PCR random mutagenesis with usage of GeneMorph II Random Mutagenesis Kit (Agilent Technologies, Inc., Santa Clara, Calif.), site-specific saturation mutagenesis of certain sites of the gene encoding the thiolase by QuikChange Multi Site-Directed Mutagenesis Kit (Agilent Technologies, Inc., Santa Clara, Calif., USA) and DNA shuffling of the gene encoding thiolase by JBS DNA-Shuffling Kit (Jena Bioscience GmbH, Jena, Germany). .. These genes are amplified from their vectors in the library through PCR using appropriate primers to append homology on each end for recombination into the vector backbone with Phusion polymerase (Thermo Scientific, Waltham, Mass.) to serve as the gene insert.

    Article Title: Biosynthesis of polyketides
    Article Snippet: .. The AraC mutant can be acquired through methods selected from error prone PCR random mutagenesis with usage of GeneMorph II Random Mutagenesis Kit (Agilent Technologies, Inc., Santa Clara, Calif.), site-specific saturation mutagenesis of certain sites of the gene encoding the thiolase by QuikChange Multi Site-Directed Mutagenesis Kit (Agilent Technologies) and DNA shuffling of the gene encoding thiolase by JBS DNA-Shuffling Kit (Jena Bioscience GmbH, Jena, Germany) on wild-type AraC. .. The AraC mutant can be acquired through methods selected from error prone PCR random mutagenesis with usage of GeneMorph II Random Mutagenesis Kit (Agilent Technologies, Inc., Santa Clara, Calif.), site-specific saturation mutagenesis of certain sites of the gene encoding the thiolase by QuikChange Multi Site-Directed Mutagenesis Kit (Agilent Technologies) and DNA shuffling of the gene encoding thiolase by JBS DNA-Shuffling Kit (Jena Bioscience GmbH, Jena, Germany) on wild-type AraC.



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    (A) Northern blot analyses of total RNA from C. jejuni WT and ribonuclease (RNase) deletion mutant strains in exponential phase. Colored triangles: prominent CJnc230 transcripts determined by termination <t>site</t> <t>sequencing</t> (term-seq) and/or primer extension. The flgE mRNA was detected with CSO-5136 (binding the coding sequence (CDS)), CJnc230 sRNA was probed with CSO-0537 (middle of sRNA) or CSO-4297 (3’-extended versions, binding ∼70 nt downstream of annotated 3’ end ). RnpB RNA (CSO-0497) served as a loading control. Oligonucleotide binding positions are indicated in ( C ). (B) Primer extension analysis of CJnc230 5’ ends from C. jejuni WT and RNase deletion mutant strains in exponential phase using the CJnc230 probe used for northern blots (CSO-0537) binding in the middle of the sRNA. A sequencing ladder generated with this probe is partially indicated on the left. Black triangle: CJnc230 5’ end in the WT (C residue in bold on the left). Gray triangle: alternative 5’ end 28 nt upstream in Δ rnc (T residue in bold). (C) Term-seq and total RNA-seq cDNA coverages at the CJnc230 locus in C. jejuni WT and nuclease deletion mutants grown to exponential phase. One representative replicate each is shown. For term-seq libraries, coverage for the last base of each read was plotted (3’nt), while total RNA-seq tracks depict full read coverage. Bent arrow: metB transcriptional start site (TSS) from . Dashed arrows: prominent CJnc230 3’ ends (T 1 , T 2 , and T 3 ) with resulting sRNA lengths in nucleotides and RNA 3’ end downstream of flgE (T flgE ). Oligonucleotide binding positions used for northern blot in ( A ) are indicated. (D) Reverse transcription-polymerase chain reaction (RT-PCR) analysis of total RNA from C. jejuni WT, Δ rnc , and Δ rny harvested at exponential growth phase. ( Left ) The flgE -CJnc230- metB locus. Arrows below: RT-PCR primers. Bent arrows: TSSs & promoter motifs ( ; ). ( Right ) Agarose gels of RT-PCR reactions performed in the presence (+) or absence (-) of reverse transcriptase (RT). Reactions with genomic <t>DNA</t> (gDNA) of WT or water (H 2 O) served as positive and negative controls, respectively. (E) Model of CJnc230 processing and maturation by three ribonucleases. RNase III potentially cleaves a predicted stem-loop in the flgE -CJnc230 transcript ( Fig. S3 ), while RNase Y and PNPase are involved in maturation of the sRNA 3’ end (full-length CJnc230, 98 nt, colored in black) or in degradation of precursor transcripts.
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    Image Search Results


    (A) Northern blot analyses of total RNA from C. jejuni WT and ribonuclease (RNase) deletion mutant strains in exponential phase. Colored triangles: prominent CJnc230 transcripts determined by termination site sequencing (term-seq) and/or primer extension. The flgE mRNA was detected with CSO-5136 (binding the coding sequence (CDS)), CJnc230 sRNA was probed with CSO-0537 (middle of sRNA) or CSO-4297 (3’-extended versions, binding ∼70 nt downstream of annotated 3’ end ). RnpB RNA (CSO-0497) served as a loading control. Oligonucleotide binding positions are indicated in ( C ). (B) Primer extension analysis of CJnc230 5’ ends from C. jejuni WT and RNase deletion mutant strains in exponential phase using the CJnc230 probe used for northern blots (CSO-0537) binding in the middle of the sRNA. A sequencing ladder generated with this probe is partially indicated on the left. Black triangle: CJnc230 5’ end in the WT (C residue in bold on the left). Gray triangle: alternative 5’ end 28 nt upstream in Δ rnc (T residue in bold). (C) Term-seq and total RNA-seq cDNA coverages at the CJnc230 locus in C. jejuni WT and nuclease deletion mutants grown to exponential phase. One representative replicate each is shown. For term-seq libraries, coverage for the last base of each read was plotted (3’nt), while total RNA-seq tracks depict full read coverage. Bent arrow: metB transcriptional start site (TSS) from . Dashed arrows: prominent CJnc230 3’ ends (T 1 , T 2 , and T 3 ) with resulting sRNA lengths in nucleotides and RNA 3’ end downstream of flgE (T flgE ). Oligonucleotide binding positions used for northern blot in ( A ) are indicated. (D) Reverse transcription-polymerase chain reaction (RT-PCR) analysis of total RNA from C. jejuni WT, Δ rnc , and Δ rny harvested at exponential growth phase. ( Left ) The flgE -CJnc230- metB locus. Arrows below: RT-PCR primers. Bent arrows: TSSs & promoter motifs ( ; ). ( Right ) Agarose gels of RT-PCR reactions performed in the presence (+) or absence (-) of reverse transcriptase (RT). Reactions with genomic DNA (gDNA) of WT or water (H 2 O) served as positive and negative controls, respectively. (E) Model of CJnc230 processing and maturation by three ribonucleases. RNase III potentially cleaves a predicted stem-loop in the flgE -CJnc230 transcript ( Fig. S3 ), while RNase Y and PNPase are involved in maturation of the sRNA 3’ end (full-length CJnc230, 98 nt, colored in black) or in degradation of precursor transcripts.

    Journal: bioRxiv

    Article Title: Interplay of two small RNAs fine-tunes hierarchical flagellar gene expression in the foodborne pathogen Campylobacter jejuni

    doi: 10.1101/2023.04.21.537696

    Figure Lengend Snippet: (A) Northern blot analyses of total RNA from C. jejuni WT and ribonuclease (RNase) deletion mutant strains in exponential phase. Colored triangles: prominent CJnc230 transcripts determined by termination site sequencing (term-seq) and/or primer extension. The flgE mRNA was detected with CSO-5136 (binding the coding sequence (CDS)), CJnc230 sRNA was probed with CSO-0537 (middle of sRNA) or CSO-4297 (3’-extended versions, binding ∼70 nt downstream of annotated 3’ end ). RnpB RNA (CSO-0497) served as a loading control. Oligonucleotide binding positions are indicated in ( C ). (B) Primer extension analysis of CJnc230 5’ ends from C. jejuni WT and RNase deletion mutant strains in exponential phase using the CJnc230 probe used for northern blots (CSO-0537) binding in the middle of the sRNA. A sequencing ladder generated with this probe is partially indicated on the left. Black triangle: CJnc230 5’ end in the WT (C residue in bold on the left). Gray triangle: alternative 5’ end 28 nt upstream in Δ rnc (T residue in bold). (C) Term-seq and total RNA-seq cDNA coverages at the CJnc230 locus in C. jejuni WT and nuclease deletion mutants grown to exponential phase. One representative replicate each is shown. For term-seq libraries, coverage for the last base of each read was plotted (3’nt), while total RNA-seq tracks depict full read coverage. Bent arrow: metB transcriptional start site (TSS) from . Dashed arrows: prominent CJnc230 3’ ends (T 1 , T 2 , and T 3 ) with resulting sRNA lengths in nucleotides and RNA 3’ end downstream of flgE (T flgE ). Oligonucleotide binding positions used for northern blot in ( A ) are indicated. (D) Reverse transcription-polymerase chain reaction (RT-PCR) analysis of total RNA from C. jejuni WT, Δ rnc , and Δ rny harvested at exponential growth phase. ( Left ) The flgE -CJnc230- metB locus. Arrows below: RT-PCR primers. Bent arrows: TSSs & promoter motifs ( ; ). ( Right ) Agarose gels of RT-PCR reactions performed in the presence (+) or absence (-) of reverse transcriptase (RT). Reactions with genomic DNA (gDNA) of WT or water (H 2 O) served as positive and negative controls, respectively. (E) Model of CJnc230 processing and maturation by three ribonucleases. RNase III potentially cleaves a predicted stem-loop in the flgE -CJnc230 transcript ( Fig. S3 ), while RNase Y and PNPase are involved in maturation of the sRNA 3’ end (full-length CJnc230, 98 nt, colored in black) or in degradation of precursor transcripts.

    Article Snippet: A sequencing ladder was generated using the DNA Cycle Sequencing kit (Jena Bioscience) according to the manufacturer’s recommendations with the CJnc230 sRNA region amplified from NCTC11168 WT (CSS-5295) gDNA using oligonucleotides CSO-3995 x 3993.

    Techniques: Northern Blot, Mutagenesis, Sequencing, Binding Assay, Generated, RNA Sequencing Assay, Reverse Transcription Polymerase Chain Reaction